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SEQUENCE ANALYSIS, DNA --- MOLECULAR SEQUENCE DATA --- CLONING, MOLECULAR --- GENETIC TECHNIQUES --- GENES, BACTERIAL --- GENES, PROTOZOAN --- GENETICS, MICROBIAL --- VACCINATION --- STANDARDS

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RT-PCR, the most widely used technique in biomedical research for analyzing gene expression, has proven a significant boon to the molecular investigation of disease pathogenesis. In RT-PCR Protocols, Joe O'Connell has assembled a panel of highly regarded molecular biologists and clinical researchers to describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering. Each protocol emphasizes the technical steps critical for experimental success and includes tips on avoiding potential pitfalls, notes explaining the method's scientific basis and its possible sources of error, and comments on how it might best be accommodated to various experimental circumstances. Diverse and wide-ranging, RT-PCR Protocols offers investigators powerful techniques to be used in the molecular investigation of disease pathogenesis and shows how RT-PCR can complement other technological advances in the design of today's new therapeutic strategies.

Polymerase chain reaction --- Réaction en chaîne de la polymérase --- Laboratory manuals. --- Manuels de laboratoire --- Electronic books. -- local. --- Polymerase chain reaction -- Laboratory manuals. --- Polymerization. --- Reverse Transcriptase Polymerase Chain Reaction --- Polymerase Chain Reaction --- Nucleic Acid Amplification Techniques --- Genetic Techniques --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- Réaction en chaîne de la polymérase --- Polymerisation --- Polymers --- Polymers and polymerization --- Synthesis of polymers --- Chemical reactions --- Synthesis --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Polymerase chain reaction - Laboratory manuals

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This volume and its companion, Volume 351, are specifically designed to meet the needs of graduate students and postdoctoral students as well as researchers, by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines. Specific topics addressed in this book include basic techniques, making mutants, genomics, and proteomics.

Genetic Techniques. --- Molecular Biology. --- Yeasts --- 582.282.23 --- 575 --- 577.2 --- Biochemical Genetics --- Biology, Molecular --- Genetics, Biochemical --- Genetics, Molecular --- Molecular Genetics --- Biochemical Genetic --- Genetic, Biochemical --- Genetic, Molecular --- Molecular Genetic --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- Genetic Counseling --- Genetic Processes --- genetics. --- Saccharomycetineae. Yeasts. Brettanomyces --- General genetics. General cytogenetics. Immunogenetics. Evolution. Speciation. Phylogeny --- Molecular bases of life. Molecular biology --- methods --- Molecular biology. --- Saccharomyces cerevisiae. --- Yeast. --- Yeast fungi. --- Biochemistry --- Fungi --- Investigative Techniques --- Biology --- Biological Science Disciplines --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Chemistry --- Eukaryota --- Natural Science Disciplines --- Organisms --- Disciplines and Occupations --- Genetic Techniques --- Molecular Biology --- Genetic Phenomena --- 577.2 Molecular bases of life. Molecular biology --- 575 General genetics. General cytogenetics. Immunogenetics. Evolution. Speciation. Phylogeny --- 582.282.23 Saccharomycetineae. Yeasts. Brettanomyces --- Yeast --- Natural Sciences --- Physical Sciences --- Discipline, Natural Science --- Disciplines, Natural Science --- Natural Science --- Natural Science Discipline --- Physical Science --- Science, Natural --- Science, Physical --- Sciences, Natural --- Sciences, Physical --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Biologic Sciences --- Biological Science --- Science, Biological --- Sciences, Biological --- Biological Sciences --- Life Sciences --- Biologic Science --- Biological Science Discipline --- Discipline, Biological Science --- Disciplines, Biological Science --- Life Science --- Science Discipline, Biological --- Science Disciplines, Biological --- Science, Biologic --- Science, Life --- Sciences, Biologic --- Sciences, Life --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Fungi, Filamentous --- Molds --- Filamentous Fungi --- Filamentous Fungus --- Fungus --- Fungus, Filamentous --- Mold --- Fungal Proteins --- Genetic Structures --- Allergens --- Microbiology --- genetics --- Genomics. --- Cell Biology.

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Computational and Statistical Genomics aims to help researchers deal with current genomic challenges. Topics covered include: overviews of the role of supercomputers in genomics research, the existing challenges and directions in image processing for microarray technology, and web-based tools for microarray data analysis; approaches to the global modeling and analysis of gene regulatory networks and transcriptional control, using methods, theories, and tools from signal processing, machine learning, information theory, and control theory; state-of-the-art tools in Boolean function theory, time-frequency analysis, pattern recognition, and unsupervised learning, applied to cancer classification, identification of biologically active sites, and visualization of gene expression data; crucial issues associated with statistical analysis of microarray data, statistics and stochastic analysis of gene expression levels in a single cell, statistically sound design of microarray studies and experiments; and biological and medical implications of genomics research.

Genomics --- DNA microarrays --- Microarray Analysis --- Sequence Analysis --- Models, Biological --- Molecular Probe Techniques --- Computational Biology --- Genetics --- Nucleic Acid Hybridization --- Genetic Techniques --- Microchip Analytical Procedures --- Biology --- Models, Theoretical --- Investigative Techniques --- Biological Science Disciplines --- Chemistry Techniques, Analytical --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Natural Science Disciplines --- Disciplines and Occupations --- Oligonucleotide Array Sequence Analysis --- Models, Genetic --- Health & Biological Sciences --- Mathematical models --- Statistical methods --- Data processing --- Animal anatomy. --- Cancer research. --- Biotechnology. --- Signal processing. --- Image processing. --- Speech processing systems. --- Statistics . --- Animal Anatomy / Morphology / Histology. --- Cancer Research. --- Signal, Image and Speech Processing. --- Statistics, general. --- Statistical analysis --- Statistical data --- Statistical science --- Mathematics --- Econometrics --- Computational linguistics --- Electronic systems --- Information theory --- Modulation theory --- Oral communication --- Speech --- Telecommunication --- Singing voice synthesizers --- Pictorial data processing --- Picture processing --- Processing, Image --- Imaging systems --- Optical data processing --- Processing, Signal --- Information measurement --- Signal theory (Telecommunication) --- Chemical engineering --- Genetic engineering --- Cancer research --- Animal anatomy --- Animals --- Physiology --- Anatomy --- Mathematical models. --- Data processing. --- Statistical methods.

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today?truly an essential publication for researchers in all fields of life sciences.Major Sections Include: * Basic Principles, Specialized Uses, and Genetic Applications* LC

Dissection. --- Lasers in biology. --- Microdissection. --- Microscopy. --- Surgical Procedures, Operative --- Specimen Handling --- Investigative Techniques --- Microscopy --- Optical Devices --- Radiation Equipment and Supplies --- Equipment and Supplies --- Diagnostic Imaging --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Laboratory Techniques and Procedures --- Diagnosis --- Diagnostic Techniques and Procedures --- Dissection --- Microscopy, Confocal --- Lasers --- Genetic Techniques --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Continuous Wave Lasers --- Pulsed Lasers --- Q-Switched Lasers --- Masers --- Continuous Wave Laser --- Laser --- Laser, Continuous Wave --- Laser, Pulsed --- Laser, Q-Switched --- Lasers, Continuous Wave --- Lasers, Pulsed --- Lasers, Q-Switched --- Maser --- Pulsed Laser --- Q Switched Lasers --- Q-Switched Laser --- Confocal Laser Scanning Microscopy --- Confocal Microscopy --- Confocal Microscopy, Scanning Laser --- Laser Microscopy --- Laser Scanning Confocal Microscopy --- Laser Scanning Microscopy --- Microscopy, Confocal, Laser Scanning --- Confocal Microscopies --- Laser Microscopies --- Laser Scanning Microscopies --- Microscopies, Confocal --- Microscopies, Laser --- Microscopies, Laser Scanning --- Microscopy, Laser --- Microscopy, Laser Scanning --- Scanning Microscopies, Laser --- Scanning Microscopy, Laser --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- Dissections --- Diagnostic Technics and Procedures --- Technics and Procedures, Diagnostic --- Techniques and Procedures, Diagnostic --- Imaging, Diagnostic --- Imaging, Medical --- Medical Imaging --- Device, Medical --- Devices, Medical --- Equipment --- Inventories --- Medical Device --- Supplies --- Apparatus and Instruments --- Devices --- Medical Devices --- Device --- Instruments and Apparatus --- Inventory --- Supplies and Equipment --- Optical Components --- Optical System --- Component, Optical --- Components, Optical --- Device, Optical --- Devices, Optical --- Optical Component --- Optical Device --- Optical Systems --- System, Optical --- Systems, Optical --- Microscopies --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Specimen Collection --- Collection, Specimen --- Collections, Specimen --- Handling, Specimen --- Handlings, Specimen --- Specimen Collections --- Specimen Handlings --- Ghost Surgery --- Operative Procedures --- Operative Surgical Procedure --- Operative Surgical Procedures --- Procedure, Operative Surgical --- Procedures, Operative Surgical --- Surgery, Ghost --- Surgical Procedure, Operative --- Operative Procedure --- Procedure, Operative --- Procedures, Operative --- Antemortem Diagnosis --- Diagnoses and Examinations --- Examinations and Diagnoses --- Postmortem Diagnosis --- Antemortem Diagnoses --- Diagnoses --- Diagnoses, Antemortem --- Diagnoses, Postmortem --- Diagnosis, Antemortem --- Diagnosis, Postmortem --- Postmortem Diagnoses --- Disease --- methods --- diagnosis --- Optical Tweezers --- Laser Therapy --- Genetic Counseling --- Genetic Phenomena --- Anatomy --- Sensitivity and Specificity --- Radiologic and Imaging Nursing --- Image Processing, Computer-Assisted --- Optics and Photonics --- Compound Microscopy --- Hand-Held Microscopy --- Light Microscopy --- Optical Microscopy --- Simple Microscopy --- Hand Held Microscopy --- Microscopy, Compound --- Microscopy, Hand-Held --- Microscopy, Light --- Microscopy, Optical --- Microscopy, Simple --- General Surgery --- Diagnostic Testing --- Testing, Diagnostic --- Diagnose --- Surgical Procedures --- Procedure, Surgical --- Procedures, Surgical --- Surgical Procedure --- Genetic Techniques. --- Lasers. --- methods.